IC50 Calculator for DPPH Assay
Introduction & Importance
Calculating the IC50 value for DPPH assay is crucial in determining the effectiveness of antioxidants. Learn how with our calculator and guide.
How to Use This Calculator
- Enter the concentration, absorbance, and control absorbance values.
- Click ‘Calculate’.
- View the IC50 value and chart below.
Formula & Methodology
The IC50 value is calculated using the formula: IC50 = [Concentration] / (1 – [Absorbance] / [Control Absorbance]).
Real-World Examples
Data & Statistics
| Antioxidant | IC50 (µM) |
|---|---|
| Vitamin C | 20.5 |
| Resveratrol | 15.3 |
| Concentration (µM) | Absorbance | Control Absorbance | IC50 (µM) |
|---|---|---|---|
| 50 | 0.25 | 0.5 | 39.2 |
| 100 | 0.42 | 0.5 | 23.8 |
Expert Tips
- Always use fresh DPPH solution for accurate results.
- Ensure the control absorbance is around 0.7-0.9 for reliable IC50 calculations.
- Consider using a microplate reader for high-throughput DPPH assays.
Interactive FAQ
What is DPPH?
DPPH is a stable free radical used to measure antioxidant activity.
Why is IC50 important?
IC50 indicates the concentration of an antioxidant required to scavenge 50% of DPPH radicals, providing a quantitative measure of antioxidant potency.